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Preparation for SAGC

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KeystoneCops
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Preparation for SAGC

Summary:

I’m going to reverse a female autoflowering plant to harvest gynoecious (XX) pollen for making gynoecious (feminized) seeds.

Plan:

I’m going to germinate 1 seed in a soil-filled solo cup. I’ll use Happy Frog.

Once the plant is ready I’ll transplant into a #300 pot filled with water-only soil.

I’m going to apply the reversal spray at week 2 or 3. Fingers crossed that it takes.

 

Grow Space:

I’m going to start the plant in my vegetative tent where I have other seedlings. I’m probably going to use my 15”x15” box with an HLG 65 4k. Males are simple in that sense.

 

Goal:

I want to collect pollen before the Spring Autoflower Grow Challenge begins, and I want to do this without pollinating my other plants.

Let’s Goooooooooo!

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Posted : 03/05/2022 4:47 pm
KeystoneCops
Prominent Member
Cannabis Fan
Completed Grows
Preparation for SAGC

I had the seed in a 16oz solo cup of coco. I very carefully removed it and placed it in a rapid rooter. I did this after realizing I’ve been overwatering my seedlings in coco, something I didn’t used to do in ProMix.

The seed has germinated.

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Posted : 03/07/2022 9:55 am
KeystoneCops
Prominent Member
Cannabis Fan
Completed Grows
Preparation for SAGC

Cotyledon have emerged with a bit of shell attached. 

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Posted : 03/08/2022 11:33 am
KeystoneCops
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Completed Grows
Preparation for SAGC

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Normally I try not to interfere with seedlings as they shed their shells, but the cotyledon where stuck closed by a dried membrane, so I very carefully liberated them.

The taproot was sticking out of the rapid rooter, so I transplanted it into a solo cup of soil. I watered it in a little, and covered it in a clear plastic cup.

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Posted : 03/09/2022 10:02 am
KeystoneCops
Prominent Member
Cannabis Fan
Completed Grows
Preparation for SAGC

First serrated leaves are visible but still small. I gave the plant a shot glass of water; soil was quite hydrophobic. I’m going to add yucca and mosquito bits next time.

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Posted : 03/10/2022 3:07 pm
KeystoneCops
Prominent Member
Cannabis Fan
Completed Grows
Preparation for SAGC

Second set of serrated leaves are small but visible. I removed the clear cup and watered with yucca and mosquito bits.

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Posted : 03/13/2022 1:29 pm
KeystoneCops
Prominent Member
Cannabis Fan
Completed Grows
Preparation for SAGC

I decided to use my 2’x2’x4’ tent and an old “blurple” lamp to grow this reversed pollen donor. A family member will host this plant, so the setup makes the most sense.

I’ll be using an in-line AC Infinity Raxial 4” fan and a 4” VanLeno carbon filter to keep pollen and odors to a minimum. I’m going to finish setting up tonight/tomorrow and move the plant over soon.

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Posted : 03/15/2022 2:48 pm

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Watered with a healthy dose of Recharge to ensure the soil has a thriving rhizosphere.

I punched a lot more holes into a spare solo cup and swapped the plant to the airier pot. Roots looked healthy, but I didn’t spend a lot of time inspecting them.

I should apply the reversing agent soon, since it goes on 2 weeks before the plant heads into bloom. 

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Posted : 03/23/2022 7:04 am

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Posted : 03/24/2022 1:46 pm

I applied the reversal agent to the plant tonight. I left it in darkness for 4 hours.

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Posted : 03/26/2022 7:07 pm

I see female flowers. That doesn’t mean the reversal agent failed. These are normal “pre-flowers.”

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Posted : 04/01/2022 3:02 am New grower liked

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I can’t be certain, but I have my fingers crossed that the reversal process worked.

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Posted : 04/06/2022 6:16 pm

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Documenting the changes day by day.

In my opinion, there are staminate flowers present. Until anthers appear, I can’t be certain. Furthermore, until I actually see seeds in the target females I won’t know if XX pollen is viable.

It’s totally possible this plant is in bloom; I don’t have enough experience with autoflowering plants to say for certain. I think so, but also it doesn’t really matter because it’ll be clear in retrospect and with autos it’s about overall cycle time (IMHO).

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Posted : 04/07/2022 3:55 pm
KeystoneCops
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Day: 35
Flower Day: 5

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There are 100% staminate flowers present. Hopefully the plant gets a lot bigger before it’s done, but this cluster alone will produce more than enough pollen.

If you’re thinking of making pollen, consider wearing an N95 mask when handling it.

Cannabis Grow CF1C201F 60FB 41FF A614 0F08EF7F4A4F

I don’t have a natural light source in the grow space. This is the overall view of the  plant. Rather tiny.

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Posted : 04/09/2022 4:16 pm
KeystoneCops
Prominent Member
Cannabis Fan
Completed Grows
Day: 47
Flower Day: 17

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I made this slotted plate for collecting pollen. I think I’d like to make it out of glass  instead, but lexan worked as a proof of concept.

I collected a very small amount of pollen (probably only a few million spores) but I expect a lot more to drop in the next few days.

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Posted : 04/20/2022 5:43 pm
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